IMAGE: Design of the majority PNIPAAm nanopore-patterned floor. (A) Diagram displaying the three-step nanoreplication course of consisting of aluminum anodizing, nickel nanoelectroforming, and UV nanoimprinting. (B) SEM photos (scale bar: 500 nm)…
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Credit score: POSTECH

Stem cells are cell factories that consistently divide themselves to create new cells. Implanting stem cells in broken organs can regenerate new tissues. Cell sheet engineering, which permits stem cells to be transplanted into broken areas within the type of sheets made up of solely cells, fully eliminates immune rejection attributable to exterior substances and encourages tissue regeneration. A analysis workforce led by POSTECH lately succeeded in drastically decreasing the harvest interval of such stem cell sheets.

A joint analysis workforce comprised of Professor Dong Sung Kim and researcher Andrew Choi of POSTECH’s Division of Mechanical Engineering and Dr. InHyeok Rhyou and Dr. Ji-Ho Lee of the Division of Orthopedic Surgical procedure at Pohang Semyung Christianity Hospital has considerably decreased the whole harvest interval of a stem cell sheet to 2 days. The nanotopography of poly(N-isopropylacrylamide) (PNIPAAm), which abruptly adjustments its roughness relying on temperature, permits harvesting of cell sheets that encompass mesenchymal stem cells derived from human bone marrow. Contemplating that it takes one week on common to make stem cells into sheets utilizing the prevailing strategies developed to this point, that is the shortest harvest time on document. These analysis findings have been revealed as a canopy paper within the newest situation of Biomaterials Science, a world journal within the biomaterials discipline.

Professor Kim’s analysis workforce centered on PNIPAAm, a polymer that both combines with water or averts it relying on the temperature. In earlier research, PNIPAAm has been launched as a coating materials for cell tradition platform to reap cell sheets, however the vary of utilization had been hampered because of the restricted forms of cells that may be made into sheets. For the primary time in 2019, the analysis workforce developed a know-how of simply regulating the roughness of 3D bulk PNIPAAm and has stably produced varied forms of cells into sheets.

The examine performed this time centered on making stem cells – which are efficient in tissue regeneration – into sheets in a short while with a view to improve their direct utility. The workforce achieved this by making use of an isotropic sample of nanopores measuring 400 nanometers (nm, 1 billionth of a meter) on the floor of a 3D bulk PNIPAAm. Consequently, not solely did the formation and maturity of human bone marrow-derived mesenchymal stem cells on the nanotopography of bulk PNIPAAm speed up, however the floor roughness of bulk PNIPAAm at room temperature beneath the decrease vital resolution temperature (LCST) was additionally quickly elevated, successfully inducing the detachment of cell sheets. This in flip enabled the fast harvesting of human bone marrow-derived mesenchymal stem cell sheets.

“At the very least 5 days are wanted to reap stem cell sheets reported by means of earlier researches,” commented Andrew Choi, the ” writer of the paper. “We will now harvest them in simply two days with the PNIPAAm nanotopography developed this time.”

“We’ve got considerably shortened the harvest time by introducing nanotopography on the floor of the 3D bulk PNIPAAm to provide mature stem cell sheets for the primary time on the earth,” remarked Professor Dong Sung Kim who led the examine. He added, “We’ve got opened up the opportunity of making use of the sheets on to sufferers sooner or later.”


The analysis was performed with the assist from Primary Analysis Program (Mid-career Researcher Program) and the Biomedical Know-how Growth Program of the Nationwide Analysis Basis and the Ministry of Science and ICT of Korea.

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